Biosafety Cabinet

Types of Bio safety Cabinets :
Class I Biosafety Cabinet :
Protection : Provides protection for the user and the environment but not for the sample.

Airflow : Air is drawn into the cabinet and filtered before being exhausted outside the laboratory.

Application : Used for handling low to moderate-risk biological agents that do not require containment.
Class II Bio safety Cabinet :
Protection : Provides protection for the user, the environment, and the sample.

Airflow : A combination of laminar flow and HEPA (High-Efficiency Particulate Air) filtration systems. Air is drawn into the cabinet, passed through a HEPA filter, and then recirculated or exhausted.

Sub types :

Class II Type A1/A2 : Recirculates air within the cabinet but may be ducted for external exhaust.
Class II Type B1/B2 : Primarily designed for use with volatile chemicals and radioactive materials, with Type B2 providing 100% external exhaust.

Application : Suitable for working with low to moderate-risk biological agents that require containment and protection.
Class III Biosafety Cabinet :
Protection : Provides the highest level of protection for the user, the environment, and the sample. It is completely enclosed with a negative pressure system and is often referred to as a “glove box.”

Airflow : All air is filtered through HEPA filters before being exhausted. The cabinet is sealed to prevent any escape of contaminants.

Application : Used for handling high-risk biological agents and for work that requires maximum containment, such as work with select agents and toxins.